Reporter

Part:BBa_K2150015:Design

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pTet TetX-GFP(fusion protein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1939


Design Notes

The fusion protein can accurately report the expression level of TetX with fluorescence intensity of GFP. PTet is used to test the expression of tetX at the presence of tetracycline conveniently.


Source

PTet comes from BBa_R0040,RBS from BBa_B0034,D.Ter from BBa_B0015. As for fusion protein tetX-GFP, tetX is the same as (K2150101), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).

References